Based on CellTiter-Glo® Luminescent Cell Viability Assay (Promega, G7570) technology, compounds with significant inhibitory effects on human bladder cancer T24 cells were screened from 1920 Chinese medicine extract libraries.

We use Promega's Cell-titer glo kit and use a 384-well plate (Corning, 3765) mode for testing. The initial screening uses each compound in a single concentration, single-well format. Drug library selection 51.KIB Natural Products library 1#, which contains 1920 kinds of natural compounds and some natural derivatives derived from medicinal plants, etc. provided by the State Key Laboratory of Phytochemistry and Sustainable Utilization of Western Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences.

The preliminary screening results indicated that the cell viablility inhibition rate of 26 hits reached 50%. The re-screening results indicated that the cell activity inhibition rate of 23 hits have reached 50%. The 23 positive results obtained, the inhibition rate ranged from 99.2% to 76.6%, and the molecular weight ranged from 258 to 1064 (average 707).

In our study, 23 compounds with significant inhibitory effects on human bladder cancer T24 cells were screened from 1920 Chinese medicine extract libraries. In the follow-up study, we will conduct IC50 determination, pharmacological and toxicological verification of these 23 traditional Chinese medicine extracts, and study the molecular mechanism of inhibiting bladder proliferation.