Screening of traditional Chinese medicines specifically inhibiting the proliferation of bladder cancer T24 cells
Number:27ESURABS0008
Type:Scientific Electronic Presentation
Authors: Kun Pang, Lin Hao, Zhenduo Shi, Conghui Han
Keywords:traditional Chinese medicines,Screen,bladder cancer,proliferation
SECTIONS
Objectives

Based on CellTiter-Glo® Luminescent Cell Viability Assay (Promega, G7570) technology, compounds with significant inhibitory effects on human bladder cancer T24 cells were screened from 1920 Chinese medicine extract libraries.


Material and methods

We use Promega's Cell-titer glo kit and use a 384-well plate (Corning, 3765) mode for testing. The initial screening uses each compound in a single concentration, single-well format. Drug library selection 51.KIB Natural Products library 1#, which contains 1920 kinds of natural compounds and some natural derivatives derived from medicinal plants, etc. provided by the State Key Laboratory of Phytochemistry and Sustainable Utilization of Western Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences.


Results

The preliminary screening results indicated that the cell viablility inhibition rate of 26 hits reached 50%. The re-screening results indicated that the cell activity inhibition rate of 23 hits have reached 50%. The 23 positive results obtained, the inhibition rate ranged from 99.2% to 76.6%, and the molecular weight ranged from 258 to 1064 (average 707).


Conclusions

In our study, 23 compounds with significant inhibitory effects on human bladder cancer T24 cells were screened from 1920 Chinese medicine extract libraries. In the follow-up study, we will conduct IC50 determination, pharmacological and toxicological verification of these 23 traditional Chinese medicine extracts, and study the molecular mechanism of inhibiting bladder proliferation.