Comprehensive Analysis of the Gene Expression Characteristics after Puerarin Treatment of Bladder Cancer T24 Cells
Number:27ESURABS0010
Type:Scientific Electronic Presentation
Authors: Kun Pang, Lin Hao, Zhenduo Shi, Harry Feng, Conghui Han
Keywords:Puerarin,Bladder Cancer,Gene Expression Characteristics
SECTIONS
Objectives

In this article, we referred to bio-information analysis website tools to analyze the gene expression characteristics of Puerarin affects the proliferation of BC T24 cells.


Material and methods

The IC50 of Puerarin was measured and the BC T24 cells were divided into Puerarin group and control groups. Affymetrix® gene expression profiling microarray chip were performed to get the differentially expressed gene list (DEGL) between the 2 groups. The enrichment of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were analyzed by Metascape®. TCGA analysis tools Ualcan and GeneMINIA were used to find the key gene in molecular network.


Results

The result of gene expression profiling chip showed in the DEGL that 590 genes were up-regulated and 1087 genes were down-regulated. The pathway analysis showed by Metascape® that Puerarin may affect the "cellular metal ion homeostasis", "blood vessel development" and "positive regulation of cell death" signaling pathway. Summary of enrichment analysis in DisGeNET shows that Puerarin may be involved in the "Vascular Diseases", "tumor vasculature" and "Recurrent tumor" in BC T24 cell lines. Many key genes of different signaling pathway were found by GeneMINIA.


Conclusions

Puerarin may affect apoptosis through "tumor vasculature", "blood vessel development" and "positive regulation of cell death" in bladder cancer T24 cell lines. It may be a negative effect on the proliferation of bladder malignant tumor.