Tandem Mass Tags Based Protein quantitative Analysis of Puerarin Treatment in Bladder Cancer T24 Cells
Number:27ESURABS0011
Type:Scientific Electronic Presentation
Authors: Kun Pang, Zhenduo Shi, Lin Hao, Harry Feng, Conghui Han
Keywords:Puerarin,Bladder Cancer,Tandem Mass Tags,Protein quantitative Analysis
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Objectives

In this article, we referred to Tandem Mass Tags (TMT) Based Protein Quantification and followed by bio-information analysis website tools to analyze the protein expression characteristics after Puerarin treatment of bladder cancer T24 cells.


Material and methods

The IC50 of Puerarin was measured and the BC T24 cells were divided into Puerarin group and control groups.  TMT Based Protein Quantification were performed to get the differentially expressed protein list (DEPL) between the 2 groups. The enrichment of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were analyzed by Metascape®, protein-protein interaction (PPI) were analyzed by String® and visualized by Cytoscape®. Then TCGA database analysis tool Ualcan was used for key protein screening.


Results

The result of TMT showed in the DEPL that 793 proteins were up-regulated and 592 proteins were down-regulated. The pathway analysis showed by Metascape® that Puerarin may affect the "Cell Cycle", "Retinoblastoma gene in cancer" and "mitotic cell cycle process" signaling pathway. Summary of enrichment analysis in DisGeNET shows that Puerarin may be involved in the "DNA repair", "Transcriptional Regulation by TP53" and "Vesicle-mediated transport" in BC T24 cell lines. PPI shows that Puerarin may involved in "Malignant Glioma", "Malignant Head and Neck Neoplasm", "Meningioma", "Head and Neck Carcinoma" and "Carcinoma, Transitional Cell". The network key gene screening results finds a list of 20 proteins that were affacted by Puerarin and may involved in the proliferation of BC T24 cells.


Conclusions

Puerarin may affect apoptosis through in bladder cancer T24 cell lines. It may be an activation effect on the proliferation of bladder malignant tumor.